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KMID : 0043320100330020215
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Archives of Pharmacal Research
2010 Volume.33 No. 2 p.215 ~ p.223
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Bee Venom Protects Hepatocytes from Tumor Necrosis Factor-α and Actinomycin D
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Park Kwan-Kyu
Park Ji-Hyun
Kim Kyung-Hyun
Kim Soo-Jung
Lee Woo-Ram
Lee Kwang-Gill
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Abstract
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Honeybee (Apis mellifera) venom (BV) has a broad array of therapeutic applications in traditional medicine to treat variety of diseases. It is also known that BV possesses anti-inflammatory and anticancer effect and that it can inhibit proliferation and induces apoptosis in cancer cells, but there is no evidence of information regarding anti-apoptosis of BV on hepatocytes. In the present study, we investigated the anti-apoptotic effect of BV on tumor necrosis factor (TNF)-α with actinomycin (Act) D induces apoptosis in hepatocytes. TNF-α/Act D-treated hepatocytes were exposed to different low concentration (1, 10 and 100 ng/mL) of BV. Our results showed statistically significant inhibition in DNA damage caused by BV treatment compared to corresponding TNF-α/Act D-treated hepatocytes. BV suppressed TNF-α/Act Dtreated activation of bcl-2 family and caspase family, which resulted in inhibition of cytochrome c release and PARP cleavage. These results demonstrate that low concentration BV possess a potent suppressive effect on anti-apoptotic responses of TNF-α/Act D-treated hepatocytes and suggest that these compounds may contribute substantial therapeutic potential for the treatment of liver diseases.
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KEYWORD
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Bee venom, Caspase, Bcl-2, TNF-α, Hepatocyte, Liver
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